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KMID : 0379119970250020133
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Korean Journal of Mycology
1997 Volume.25 No. 2 p.133 ~ p.142
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Cultural Condition for the Mycelial Growth of Phellinus igniarius on Chemically Defined Medium and Grains
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Abstract
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The chemical media composition and culture conditions were optimized for mycelial growth of Phellinus igniarius 26005. The method of solld-state fermentation, cultivation of basidiomycetal strains in various grains, was developed. Media composition for optimal growth of Phellinus igniarius 26005 was made of 7.0% malt extract, 0.3% bacto soytone, and 0.2% yeast extract. The optimum condition for mycelial growth was 28℃ and pH 7.0, respectively. For the mass cultivation of mycelial, the hydrated grains with cold water were put into the plastic bottle. The mycelial growth rate in the bottled grains was high in the early stage with inoculation of homogenized mycelium. The activity of mycelium was maintained by adding sterilized water in the middle of cultivation. The glucosamine content which determine the mycelial growth rate in solid material was in the order of job´s tears$gt;barley$gt;black soybean$gt;wheat$gt;malt soybean$gt;brown rice$gt;sorghum$gt;glutinous rice.
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